Synthetic and Molecular Biology
Gene Expression Plasmids
- Gene synthesis and cloning.
- PCR amplification, gene subcloning and chimera constructs.
- Epitope tagging and fusion to fluorescent proteins.
- Site-directed and random mutagenesis:single codons or functional domains.
Construction of small mutant libraries by sited-directed mutagenesis.
Construction of small mutant libraries by random or saturation mutagenesis
- Expression or genomic libraries.