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CELL BIOLOGY

Microscopy techniques

brightfield, darkfield, phase contrast, Nomarski

Real time video recording with fluorescent probes

Confocal microscopy image acquisition

Immunocytochemistry/immunofluorescence

FRAP, FLIP, FRET & FLIM techniques

 

 

Cell culture techniques

Insertion of foreign genes by cell transfection

siRNA and shRNA gene silencing

Lymphocyte and monocytes isolation from buffy coats or whole blood

Cytotoxicity assays and IC50 determination of small molecules with therapeutic interest

 

 

Flow cytometry

Cell sorting of cell populations

Multicolour analysis of PBMC subpopulations

 

Additionally, we have developed a fast and sensitive test method for detection of the different Mycoplasma strains, that is already in the BioAssays service portfolio