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MOLECULAR BIOLOGY

 

Development of gene expression vectors

Gene synthesis and cloning

Expression and purification of recombinant proteins by affinity chromatography

Nucleic acid amplification by PCR and gene subcloning

Development of chimeras: epitope tagging and fluorescent protein fusion proteins

Random and site-directed mutagenesis

Single amino acid mini-libraries generation

Point mutation mini-libraries within a protein functional domain

Site saturation mutant mini-libraries (random mutagenesis)

cDNA or genomic DNA libraries construction

Generation of shRNA vectors for stable and transient RNAi gene silencing

Epigenetics: DNA methylation analysis