Development of gene expression vectors

Gene synthesis and cloning

Expression and purification of recombinant proteins by affinity chromatography

Nucleic acid amplification by PCR and gene subcloning

Development of chimeras: epitope tagging and fluorescent protein fusion proteins

Random and site-directed mutagenesis

Single amino acid mini-libraries generation

Point mutation mini-libraries within a protein functional domain

Site saturation mutant mini-libraries (random mutagenesis)

cDNA or genomic DNA libraries construction

Generation of shRNA vectors for stable and transient RNAi gene silencing

Epigenetics: DNA methylation analysis